Sam Wortzman and Josh Moscoe started walking at 6 a.m. on Tuesday.
Wortzman tells 570 NEWS they have already travelled from Waterloo, into Breslau, and into Guelph.
The pair expect it to be a long day, figuring the 110 km trip will take them into late tonight.
Wortzman tells 570 NEWS they "Thought it was a fun idea ... and a crazy challenge to try and do it all in one day.", adding Google Maps predicts it will take 23 hours.
The pair have already raised $5,000 for the Sick Kids Hospital Foundation.